DIO's Assassins In Stardust Crusader

In Stardust Crusader, Dio resurfaces when his coffin is pulled out of the ocean's depths by a group of treasure hunters. Now referring to himself as DIO in all capitals, rather than his full name Dio Brando, he eventually obtains a Stand via a Stand Arrow. DIO then forms a new network of followers, consisting of natural Stand users and recruiting others after piercing them with one of the Arrows too. Here is the list of them:

DIO 

After becoming a vampire and later a Stand user, Dio has gathered around him many minions and is responsible for many major events of the series, from the death of Jonathan Joestar to the Joestar Family's sudden acquisition of Stand power, and his legacy persists throughout the series. His most recognizable characteristics are his boundless ambition and his love of power. After becoming a vampire, Dio's most obvious abilities are his raw strength, regeneration, blood-sucking tentacles at his fingers and near-immortality.

• Zombie/Vampire Creation: During his time as a Vampire, Dio could create Zombies either from a living human or the cadaver of the deceased. The Zombies are also strong creatures, but less so than their vampiric creator.
• Space Ripper Stingy Eyes: Creates two pressurized fluid jets from the eyes. Strong enough to cut cleanly through a stone column.
• High-level Regeneration: The body is able to live even after having vital body parts amputated. It will regenerate and the body parts can reattach themselves. Vampires can also control their detached body parts. However, Vampires can still die if their head is damaged sufficiently.
• Vaporization Freezing Technique: A freezing ability enabling vampires to freeze their own body parts and transfer the loss of energy by touch. It is able to cancel out the Ripple by freezing the blood in the user's body.
• Fusion: An ability that allows a vampire to graft parts of different organisms together. Dio displays this ability by creating animal/human hybrid zombies as pets and later grafting his disembodied head on the decapitated body of Jonathan.
• Flesh Buds: A vampire's cells can spawn these Flesh Buds and insert them into the brains of living beings to control them.
• The World: It is an exceptionally powerful Close-range Stand but boasting an effective manifestation range of approximately 10 meters. Despite requiring a close distance to inflict damage, its overwhelming physical might and near unstoppable control over time undeniably make it one of the most dangerous Stands in the entire series. 
  
• Super Strength/Senses/Speed/Precision: The World possesses phenomenal strength, senses, precision, and incredible speed. Its main offense is generally characterized by a flurry of speedy punches and the occasional kick.
• Time Stop: The World's signature power is its ability to stop time, allowing only itself, DIO and anyone with the same power, to act within the duration of stopped time.

Vanilla Ice 

Vanilla Ice is the last Vampire created by DIO and one of the penultimate Stand Users the Joestar Group faces upon infiltrating DIO's Mansion. After the group is split up, he is sent to take out the team consisting of Avdol, Polnareff, and Iggy. He is a Stand User controlling his void-based Stand ability, Cream, to battle.

• Cream: Cream is a fairly powerful short-ranged Stand, able to perform feats such as decapitation with a mere chop to the neck. However, its true power lies in its unique void ability that makes it one of the most dangerous Stands of Stardust Crusaders. 
  
• Void: Cream is able to become a portal to a void-like dimension. Anything that passes through this portal is disintegrated into nothingness, the sole exception is Vanilla Ice himself, who can use the void dimension as a hiding place.

Gray Fly 

Gray Fly is an assassin sent by DIO who attacks the group aboard a plane traveling from Tokyo to Cairo. He is a Stand User and wields Tower of Gray. Gray Fly's Stand is Tower of Gray, which takes the form of a giant and agile stag beetle capable of easily tearing flesh apart, used by him for his crimes.

• Tower of Gray: It is a small and fragile Stand. However, it is also incredibly fast and nimble, and its secondary jaw grants it unsuspected destructive power. 
  
• Tower Needle: Its defining feature is its long stinger which protrudes from its mouth which has enough force to tear flesh apart or pierce its way right through.
• High Speed: Perhaps one of its greatest traits is its amazing speed; Tower of Gray is able to easily outmaneuver very fast Stands and has great flight control.

Impostor Captain Tennille 

An unnamed assassin, the impostor Captain Tennille is one of the many mercenaries sent by DIO to assasinate the Joestar Group on their way to Egypt. He secretly killed the ship's real captain and assumed his identity. He is a Stand User, wielding the unique aquatic-based Dark Blue Moon.

• Dark Blue Moon: it is a marine Stand and its skills are more effective in an aquatic environment. It is probably less effective in a terrestrial environment.
  
• Razor-Sharp Scales: Dark Blue Moon can also use its scales as projectiles, throwing them against the enemy.
• Barnacles: Dark Blue Moon is capable of creating barnacles, specifically acorn barnacles. The barnacles drain the target of its strength and also allow Dark Blue Moon slight control over the infected body parts.

Forever 

Forever is a malicious orangutan in DIO's service who confronts the group in the South China Sea aboard a freighter. He is a Stand User, possesing Strength, an artificial non-humanoid Stand capable of binding to and enchancing objects. In Forever’s case it is the ship which he captains.

• Strength: Disguised as a freighter, Strength is a very powerful Stand bound to take its enemies by surprise and it almost killed the entirety of the Joestar Group at once. 
  
• Ship Manipulation: Strength is capable of binding itself to a small boat and transforming it into a gigantic freighter. While Strength is bound to an object, Forever gets unquestionable psychic control over it.

Devo the Cursed 

Devo is an infamous Native American shaman and assassin in DIO's service, Devo ambushes Jean Pierre Polnareff in his hotel room in Singapore. He is a Stand User and uses Ebony Devil.

• Ebony Devil: This Stand is risky in that Devo must be injured by his targets first in order for the Stand to work. However, when set up, it can then ambush its victim and proves to be elusive. 
  
• Hatred Amplification: Ebony Devil's power activates once Devo is hit by his foe. It then grows more powerful depending on the hatred Devo feels towards his enemy relative to the amount of damage he's received. It can then transfer itself to something physical in order to attack, preferably a doll in its unique appearance.

Rubber Soul 

Impersonating Kakyoin, Rubber Soul isolates and attacks Jotaro and Anne in Singapore. Rubber Soul is a Stand User who wields Yellow Temperance. He was hired by DIO with the promise of earning 100 million dollars as a reward for killing Jotaro.

• Yellow Temperance: Its ability to absorb organic matter from physical contact result in one of the few Stands of the series that can be said to be physically invincible. On the other side, Yellow Temperance is limited by its own user's human vulnerabilities. 
  
• Assimilation: Yellow Temperance's primary power is the ability to assimilate organic matter, using it to grow stronger and larger. Thus, its user constantly seeks things to consume even when hidden.
• Appearance Replication: Yellow Temperance is able to alter Rubber Soul's physical appearance.
• Physical Imperviousness: It has the capacity to absorb physical punishment and impacts, acting as an armor for Rubber Soul. It can resist impacts, burning, and freezing.

Hol Horse 

Hol Horse is a mercenary wielding Emperor, a Stand that looks like, and functions similarly to, a gun. He was originally sent by DIO to confront the Joestar Group alongside J. Geil. However, after his partner is defeated first, he makes a tactical retreat, returning for two following encounters.

• Emperor: As a gun Stand, Emperor functions as a weapon for Hol Horse. Due to its straightforward nature, Hol Horse must compensate through cooperation with allies to gain the upper hand. 
  
• Homing Bullets: The characteristic ability of Emperor, and its most dangerous is its ability to freely control the trajectory of its bullets as they are also a part of the Stand. Because of this, the gun seems to have infinite ammunition. However, the bullets are still subject to limited range.
• Point-Blank Summon: One of the lesser properties of Emperor is that Hol Horse summons it directly in his hand. It allows him to perform sneak attacks.

J. Geil 

J. Geil is an assassin, the son of DIO's advisor Enya the Hag, sent alongside Hol Horse to kill the Joestar Group. He is also the murderer of Sherry Polnareff and the man Jean Pierre Polnareff seeks revenge from. J. Geil is a Stand User and wields Hanged Man.

• Hanged Man: Because it attacks from inside mirrors, Hanged Man is a terrifyingly evasive Stand almost impossible to defend oneself against. 
  
• Light Manifestation: Hanged Man is a Stand that attacks via reflections. It will appear in a reflective surface and attack opponent's reflection. The opponent will then bear the results of the attack. Hanged Man can move through any type of reflecting surface, including human eyes, at the speed of light.
• Wrist Knives: Hanged Man's two right hands can produce knives from its wrists to attack the enemy.

Nena 

Nena first appears in India alongside Hol Horse. She is a Stand User who uses Empress. Her first looks like a beautiful young Indian woman in typical Indian attire. However, this is only a disguise and Nena is truly a short, ugly and overweight woman wearing a bikini.

• Empress: Empress is a exceptional hybrid between flesh and Stand that is both dangerous and frustrating to get rid of if planted on its victim. 
  
• Parasitism: Empress must first be planted into another person through direct contact with Nena's blood. The zone splattered with blood will develop a fleshy bud. Empress will then slowly and continuously grow out of the victim's flesh. It will eventually reach a humanoid form and will constantly try to hinder, harm, or kill its host.
• Flesh Disguise: Empress grants Nena the ability to hide seamlessly inside the hollowed-out body of a previous victim and to talk with her previous voice.

ZZ 

He is another of DIO‘s assassins, who confronts the Joestar Group as they drive through the northern part of India. He is a Stand User who drives his Wheel of Fortune, a stand that is bound to a car. When his car is destroyed he proves to be a coward who only pretended to be strong.

• Wheel of Fortune: It is a powerful and versatile Stand thanks to its transformation ability, giving it complete control over the car it uses as a catalyst. 
  
• Transformation From Confidence: Wheel of Fortune's primary ability dramatically enhances a vehicle's appearance and capabilities, "allowing even a common car to transform into a more powerful car with four-wheel drive.
• Gasoline Bullets: Wheel of Fortune can shoot its own gasoline at its opponents as a high pressure scatter shot.
• Radio Communication: ZZ can hijack nearby cars' radios in order to converse with people.

Enya the Hag 

Enya holds a high station among DIO's confidantes. She is the mother of J. Geil and after her son's death, ambushes Jotaro's group herself with the help of her fog Stand: Justice. It is revealed that she had a key role in developing the Stand population when she acquired the Bow and Arrow.

• Justice: Justice is a terrifyingly powerful Stand because of its very nature and power. Despite the ridiculous way Jotaro defeated it, Justice is one of the most powerful Stands in the series due to its invulnerability as a fog Stand and ability to control anyone's body through an open wound. 
  
• Fog String Puppetry: Justice's foremost power allows it to control the bodies of others through an open wound. Justice's puppetry power is so powerful it can take control of dozens of corpses and manipulate them with precision, which can result in a large band of puppets under the control of Justice.
• Illusions: Justice can manifest incredibly realistic and detailed illusions anywhere within its range.

Steely Dan 

A Stand User using the microscopic Lovers, Steely Dan is sent to assassinate Enya the Hag and then confronts the Joestar Group in Pakistan. He exhibits a particularly cruel, petty and cowardly personality.

• Lovers: Lovers is very weak. Because of its microscopic size, Lovers is by itself very vulnerable. However, its ability to infiltrate the brain makes it nonetheless a formidable Stand that is very difficult to reach and its reach of hundreds of kilometers makes escape impossible. 
  
• Pain Synchronization: Lovers' primary ability is to enter the brain of its victim and manipulate their pain receptors. When Steely Dan feels any physical stimuli, Lovers will go berserk inside the brain and return the pain several folds to its victim, causing them agony.
• Organic Waste Clones: Once inside of someone’s brain, Lovers can take brain cells and turn them into mush for various uses.
• Spores Cultivation: Lovers uses DIO's spores to kill its victims. Thanks to its size, it can carry an individual spore inside someone's brain and then feed it with brain cells in order to make it grow and eventually destroy the brain.

Arabia Fats 

Arabia Fats is another of DIO's goons, who ambushes the Joestar Group in the middle of the Arabian desert with his Stand: Sun. His real personality is unknown. He was never shown speaking or otherwise emoting, as his only appearance had him unconscious after being struck by a rock.

• Sun: Sun is an amazingly powerful Stand with a large range of action. However, it doesn't do anything to protect its user, who must stay nearby and hide himself. 
  
• Sun Replica: Sun's foremost power is to emit light and heat at lethal levels over a large area. It raises the surrounding temperatures up to 80 °C.
• Lasers: In addition to its radiating of heat, Sun is also able to shoot rays of energy. It will shoot rays in all directions but can also strike with precision.

Mannish Boy 

Mannish Boy is an evil genius baby and Stand user in DIO's service. He tries to murder the Joestar Group inside the nightmare-world of his Death Thirteen. He only shows his true personality when his victims fall asleep close to him, allowing him to use his Stand, Death Thirteen.

• Death Thirteen: Death Thirteen is unique in that it can only exist inside a dream world and thus requires that its victims are asleep. It is incredibly dangerous because it is certain to take people by surprise. 
  
• Dream World Control: This ability comes in two parts. When anything alive falls asleep near Mannish Boy, Death Thirteen's User, they are drawn into Death Thirteen's dream world and it has full control over this dream world. Death Thirteen is free to torment and kill anyone inside the dream with its scythe. Any injuries the person receives in the dream world are transferred to the real world, including fatal ones.

Cameo 

Cameo isolates Polnareff from the Joestar Group on a secluded island in the Red Sea while they are there to retrieve Avdol. He is one of DIO's henchmen, and the Stand User behind the genie Stand, Judgement. He was also a cowardly man who liked to toy with people's hearts and often acted as a "genie" who turned the wishes of his victim against them.

• Judgement: Judgement is a fairly powerful Stand, having above average strength and speed as far as Stands go. Its large metal body is particularly hard to damage. 
  
• False Wish Granting: Judgement's unique power is to create clay constructs out of humans' deepest desires but it will turn the wishes against its victims.

Midler 

Midler is the last of DIO's assassins the heroes encounter before reaching Egypt. She is a Stand User, wielding a long-distance shapeshifting Stand, High Priestess. She attacks the group as they travel by submarine in the Red Sea.

• High Priestess: High Priestess is a formidable opponent. Its small size and nimbleness make it difficult to strike and its claws are very dangerous. This, combined with its ability that multiplies its options, makes for a fearsome opponent. 
  
• Mineral Possession and Reformation: High Priestess possesses the ability to shapeshift and assimilate itself into any type of inorganic matter, so long as it's a material made from a mineral substance, be it rock, metal, glass, or plastic.

Kenny G. 

When the Joestar Group enters DIO's Mansion, he casts an illusion on it to make it look like a labyrinth to them but is quickly taken out.

• Tenore Sax: It is the Stand of Kenny G. It allows Kenny G. to create complex illusions. As a Stand that generates illusions and whose user was swiftly defeated, its appearance and personality, if any, are unknown.
• Illusion: Tenore Sax has the ability to generate illusions that can distort the inside of DIO's mansion, producing incomprehensibly large rooms or complex labyrinths. The Stand can replicate not only a specific area's appearance, but its composition as well.

Nukesaku 

He is a henchman of DIO that tries to attack Jotaro when the Joestar Group has defeated DIO's other minions. Having no Stand, he is used as a punching bag and unwilling guide in the mansion. As a vampire, Nukesaku has the natural ability to dislocate his body parts.

• Female Face: Nukesaku has a female face on the back of his head, along with breasts located on his back. Using the female face, he can use it to talk and make expressions.